Large-scale production of DNA sequencing templates by microtitre format PCR
نویسندگان
چکیده
منابع مشابه
Large-scale production of DNA sequencing templates by microtitre format PCR
Large scale DNA sequencing projects require gel readers with high throughput and good base-calling accuracy with as little manual intervention as possible. The automatic sequencing machines now available meet these requirements quite well, indeed, a single ABI 373A machine can read at least around 500 samples/200 000 nt per week. Thus, a full-time sequencing laboratory will need to produce thou...
متن کاملScheduling Large-Scale DNA Sequencing Applications
Large-scale network-based computing has attracted tremendous efforts from both academia and industry because it is scalable, flexible, extendable, and economic with wide-spread applications across many disciplines in science and engineering. To address scalability issues for an important class of applications, researchers proposed a divisible load scheduling theory (DLT). These applications are...
متن کاملIndexed PCR Primers Induce Template-Specific Bias in Large-Scale DNA Sequencing Studies.
Massively parallel sequencing is rapidly emerging as an efficient way to quantify biodiversity at all levels, from genetic variation and expression to ecological community assemblage. However, the number of reads produced per sequencing run far exceeds the number required per sample for many applications, compelling researchers to sequence multiple samples per run in order to maximize efficienc...
متن کاملSequencing two DNA templates in five channels by digital compression.
By applying algebraic coding methods to the Sanger dideoxynucleotide procedure, DNA sequences of two templates can be determined simultaneously in only five reactions and data channels. A 5:2 data compression is accomplished by instantaneous source coding of nucleotide sequence pairs into one set of 5-bit block codes. A general algebraic expression, 2n-1 > or = 4f, describes conditions under wh...
متن کاملSequencing of PCR-amplified DNA.
Alternatives for sequencing of PCR products essentially fall into one of two categories; generation of single-stranded DNA for sequencing or the direct sequencing of double-stranded product. Of the two alternatives, sequencing of double-stranded PCR products is likely to be of greatest immediate significance in terms of general applicability and rapidity. Double-stranded sequencing allows the u...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1993
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/21.1.173